Biology Biotechnology MCQs Chapter 9 Class 12

Created on March 02, 2022 By Ali Durrani

Biology Ch 9 Class 12 Biotechnology

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1 / 67

DNA analysis is done to

Diagnose viral infections

Identify criminals

Determine evolution

All of these

2 / 67

Gel electrophoresis is a technique:

PMC 2020
Biology
Biotechnology
Elimination Tool:

A) Employed by forensic scientist to assist in the identification of the individuals by their respective type of DNA

B) Collect all the genes found in one complete set of chromosome

C) Is a technique to separate different sizeds fragment of charge bearing polymers (proteins, RNA or DNA)

D) Grows single cell or a group of cells in a glass ware on artificial medium under aseptic conditions

3 / 67

Golden rice is a promising transgenic crop. When released for cultivation, it will help in

PMC Practice 11
Biology
Biotechnology
Elimination Tool:

A) Producing a petrollike fuel from rice

B) Alleviation of vitamin A deficiency

C) Pest resistance

D) Herbicide tolerance

4 / 67

A genomic library is a collection of

Bacterial clones

Bacteriophage clones

Viral clones

Both a and b

5 / 67

Vaccination can be done against:

NUMS 2015
Biology
Biotechnology
Elimination Tool:

A) Bacterial diseases only

B) Viral diseases only

C) Both Viral and Bacterial

D) All type of disease-causing organisms

6 / 67

The technique use of identification of criminals is called:

NTS 2018
Biology
Biotechnology
Elimination Tool:

A) Cloning

B) DNA fingerprinting

C) Restriction Analysis

D) Polymorphism

7 / 67

Liposomes are:

PMC 2021 Tested
Biology
Biotechnology
Elimination Tool:

A) All of these

B) Made of at least one lipid bilayer

C) Artificial

D) Used to deliver drugs

8 / 67

Transgenic organisms:

PMC 2020
Biology
Biotechnology
Elimination Tool:

A) Have a foreign gene inserted into them

B) Have an important role in the large scale production of medicinal products

C) Are considered beneficial to humans

D) All of the above

9 / 67

Some transgenic bacteria produce a dipeptide sweetener called

Ultrasweet

Disweet

Monosweet

Neutrasweet

10 / 67

Cloning is the production of genetically identical copies of organisms/cells by:

NUMS 2015
Biology
Biotechnology
Elimination Tool:

A) Sexual reproduction

B) Asexual reproduction

C) Both sexual and asexual

D) None of the given options

11 / 67

Chemical nature of primers used in the PCR process is _______.

UHS 2018
Biology
Biotechnology
Elimination Tool:

A) RNA

B) Protein

C) Carbohydrate

D) DNA

12 / 67

A method of DNA sequencing in which DNA threads are chemically cut into pieces of different sizes is

Sanger's method

Maxam-Gilbert method

Hamilton's method

Vortex method A

13 / 67

Which of the following characteristics makes plasmid DNA useful for researchers?:

PMC Practice 9
Biology
Biotechnology
Elimination Tool:

A) Readily incorporate cloned DNA

B) Capable of automatic replication

C) Capable of being isolated from genomic DNA

D) All of these

14 / 67

Deficiency of enzyme ______ causes combined immunodeficiency syndrome.

UHS 2018
Biology
Biotechnology
Elimination Tool:

A) Adenosine transcriptase

B) Adenosine transaminase

C) Adenosine polymerase

D) Adenosine deaminase

15 / 67

DNA polymerase enzyme for PCR is isolated from bacteria, Thermus aquaticus, because;

UHS 2019
Biology
Biotechnology
Elimination Tool:

A) It can work at high speed.

B) It can withstand high denaturation temperature.

C) It can withstand low denaturation temperature.

D) It can be used again and again.

16 / 67

Which one of the following options is NOT an example of genetic engineering?

NTS 2019
Biology
Biotechnology
Elimination Tool:

A) Insulin-producing bacteria

B) Oil eating bacteria

C) Photosynthetic bacteria

D) Metal extracting bacteria

17 / 67

Human genome is ______ times larger than any other genome sequenced so far

45

35

25

15

18 / 67

Enzyme used by the Bacteria to cut the DNA of the invading Virus for its protection is:

UHS 2018
Biology
Biotechnology
Elimination Tool:

A) Restriction Exonuclease

B) Restriction Ligase

C) Restriction Endonuclease

D) DNA polymerase

19 / 67

A single gene can be amplified by using:

NUMS 2019 Reconduct
Biology
Biotechnology
Elimination Tool:

A) DNA fingerprinting

B) Polymerase Chain Reaction

C) Gel Electrophoresis

D) Recombinant DNA technology

20 / 67

First restriction enzyme was isolated by

Kary Mullis B

Hamilton O. Smith

Maxam Gilbert

Hamilton John

21 / 67

It is a method for rapid production of a very large number of copies of a particular fragment of DNA:

PMC 2020
Biology
Biotechnology
Elimination Tool:

A) Gel electrophoresis

B) Polymerase chain reaction

C) DNA extraction

D) Recombination

22 / 67

Genetic engineering has been successfully used for producing;

UHS 2019
Biology
Biotechnology
Elimination Tool:

A) Animals, with inferior traits.

B) Transgenic mice for testing safety of polio vaccine before use in humans

C) Animals, that have the same genetic constitution.

D) None of the above

23 / 67

Passive immunity is used against:

UHS 2015
Biology
Biotechnology
Elimination Tool:

A) Malaria

B) Typhoid

C) Dengue

D) Tetanus

24 / 67

During gel electrophoresis, the gel used is:

NUMS 2020
Biology
Biotechnology
Elimination Tool:

A) Agar gel

B) Agarose gel

C) Dextran

D) Toluene gel

25 / 67

The process of determining the locus for particular biological traits includes?

PMC Practice 2
Biology
Biotechnology
Elimination Tool:

A) Replication

B) Recombination

C) Gene mapping

D) None of these options is correct

26 / 67

In prokaryotes only one type of RNA polymerase is present, while ______ types of RNA polymerase are present in eukaryotes.

NUMS 2019 Reconduct
Biology
Biotechnology
Elimination Tool:

A) Two

B) Three

C) Four

D) Five

27 / 67

Lambda phage is

Virus

Bacterium

Plasmid

Host cell

28 / 67

Which one of the following is made up of radioactively labelled nucleotides?

UHS 2015
Biology
Biotechnology
Elimination Tool:

A) Phage DNA

B) Genomic Library

C) Recombinant DNA

D) Gene Probe

29 / 67

Commonly used restriction enzyme is

pSC 101

pBR 322

EcoRI

All of these

30 / 67

Choose the term from the following which is NOT a part of gene therapy?

NTS 2019
Biology
Biotechnology
Elimination Tool:

A) Bone marrow transplant

B) Retrovirus

C) DNA Fingerprinting

D) Somatic cells

31 / 67

By PCR we mean:

UHS 2018
Biology
Biotechnology
Elimination Tool:

A) Polymerase chronic reaction

B) Polymerase chain reaction

C) Polymerase copy reaction

D) Polymerase cross reaction

32 / 67

The genetically Engineered vaccine is not available for which of the following?

PMC Practice 8
Biology
Biotechnology
Elimination Tool:

A) HAV

B) HBV

C) HCV

D) HDV

33 / 67

A single stranded nucleotide sequence that will hybridize into a certain piece of DNA is

Primer

Probe

Genome

Both a & b

34 / 67

Tobacco plants, resistant to a nematode, have been developed by the introduction of DNA that produces (in the host cells):

PMC Practice 11
Biology
Biotechnology
Elimination Tool:

A) Both sense and antisense RNA

B) A particular hormone

C) An antifeedant

D) A toxic protein

35 / 67

Which statement is not true about PCR?

Developed by Kary Mullis

Take its name from DNA polymerase

Is not very specific

Taq polymerase is used

36 / 67

In recombinant DNA technology, the copies of recombinant DNA are increased by:

NTS 2018
Biology
Biotechnology
Elimination Tool:

A) Restriction enzyme

B) Ligase

C) Selection of host with rDNA

D) Multiplication of host with rDNA

37 / 67

A weed engineered to produce a biodegradable plastic in cell granules is

. Mouse-cat cress

Mouse weed

Mouse-eared cress

None of these

38 / 67

This enzyme is used to cut DNA molecule in rDNA technology

PMC Sample 2
Biology
Biotechnology
Elimination Tool:

A) ligase

B) phosphatase

C) ribonuclease

D) restriction enzyme

39 / 67

Which of the following is the components / tools of recombinant DNA technology?

PMC 2020
Biology
Biotechnology
Elimination Tool:

A) Gene of interest

B) Molecular scissors

C) Molecular glue and expression system

D) All of the above

40 / 67

DNA made by joining pieces from two or more different sources:

UHS 2018
Biology
Biotechnology
Elimination Tool:

A) Probes

B) Restriction endonuclease

C) Mutated DNA

D) Recombinant DNA

41 / 67

Cystic fibrosis patients lack a gene that codes for a transmembrane carrier of:

NTS 2017
Biology
Biotechnology
Elimination Tool:

A) Na+ ions

B) K+ ions

C) CI- ions

D) Ca2+ ions

42 / 67

Transgenic plants are produced when foreign genes are introduced into

Mature plant embryo

Immature plant embryo

Roots

Leaves

43 / 67

Organisms that have had a foreign gene inserted into them are

Transformed organisms

Transgenic organisms

Transfigured organisms

Recombinant organisms

44 / 67

In the commercial manufacture of insulin, a human gene is inserted into which one of the following?

NTS 2013
Biology
Biotechnology
Elimination Tool:

A) A chromosome of a human cell

B) A protein molecule in a yeast cell

C) The DNA of a bacterium

D) The nucleic acid in a virus

45 / 67

Protoplasts are plant cells from which

. Cell walls are removed

Nucleus is removed

New organelles are added

Vacuoles are taken out

46 / 67

Which of the following is the manipulation of genetic material for practical purposes?

NTS 2018
Biology
Biotechnology
Elimination Tool:

A) Grafting

B) Tissue Culture

C) Genetic Engineering

D) Cell Culture

47 / 67

Which of the following is NOT true for genes used in recombinant DNA technology?

NUMS 2019 Reconduct
Biology
Biotechnology
Elimination Tool:

A) It can isolated from chromosome

B) It can be synthesized chemically

C) It can be made from protein

D) It can be made from mRNA

48 / 67

The process of replacing or supplementing the defective allele with a functional, normal allele is known as ______.

NTS 2012
Biology
Biotechnology
Elimination Tool:

A) Allele transplant

B) Physiotherapy

C) Gene therapy

D) Mutation

49 / 67

Cloning is a form of:

NUMS 2016
Biology
Biotechnology
Elimination Tool:

A) Parthenogenesis

B) Apomixis

C) Sexual reproduction

D) Asexual reproduction

50 / 67

Which of the following diseases CANNOT be cured by gene therapy?

NUMS 2019 Reconduct
Biology
Biotechnology
Elimination Tool:

A) SCID

B) Tuberculosis

C) Cystic fibrosis

D) Cancer

51 / 67

A technique in transgenic animals in which desired gene is inserted into the eggs of animal is called:

UHS 2015
Biology
Biotechnology
Elimination Tool:

A) Embryonic Stem Cell mediated Transfer

B) Microinjection

C) Retro-virus mediated gene Transfer

D) Virus vectors

52 / 67

Natural extrachromosomal circular DNA molecules earrying genes for antibiotic resistance and fertility

Recombinant DNA

Chimaeric DNA C

Lambda phage

Plasmids

53 / 67

The deficiency of which of the following cause severe combined immunodeficiency

PMC Sample 3
Biology
Biotechnology
Elimination Tool:

A) hypoxanthine-guanine transferase

B) xanthine oxidase

C) PRPP synthatase

D) adeninosine deaminase

54 / 67

Chimaeric DNA is also known as

Recombinant DNA

Complementary DNA

Vector

Plasmid

55 / 67

Which one has antibiotic resistance gene for tetracycline and ampicillin?

pSC 101

pBR 322

pCR 322

None of these

56 / 67

The Plasmid pBR322 has antibiotic resistance genes for:

UHS 2019
Biology
Biotechnology
Elimination Tool:

A) Streptomycin

B) Ampicillin and Tetracycline

C) Tetracycline and mxycycline

D) Ampicillin

57 / 67

Treatment of hereditary disorders by gene manipulations is called:

NUMS 2016
Biology
Biotechnology
Elimination Tool:

A) Biotechnology

B) Genetic engineering

C) Gene therapy

D) None of these

58 / 67

The genetically engineered vaccine is available for which of the following hepatitis virus?

PMC Practice 5
Biology
Biotechnology
Elimination Tool:

A) HBV

B) HAV

C) HCV

D) Options A and B are correct

59 / 67

The DNA that has been altered and which now contains length of nucleotides from two different organisms is called a:

PMC 2020
Biology
Biotechnology
Elimination Tool:

A) Plasmid

B) Combined DNA

C) Vector

D) Recombinant DNA

60 / 67

Recombinant DNA contains DNA from

2 different sources

3 different sources

2 same sources

3 same sources

61 / 67

The cells are held together by a Ca-pectate layer are called as:

NUMS 2016
Biology
Biotechnology
Elimination Tool:

A) Primary cell wall

B) Secondary cell wall

C) Middle lamella

D) Tertiary cell wall

62 / 67

The enzyme which seals the foreign DNA into the vector is

Restriction endonuclease

DNA polymerase

DNA ligase

DNA mutase

63 / 67

DNA fingerprinting is basically done for:

NUMS 2015
Biology
Biotechnology
Elimination Tool:

A) DNA cloning

B) DNA analysis

C) DNA sequencing

D) DNA splicing

64 / 67

Which of the following is not necessary for PCR to occur?

PMC 2020
Biology
Biotechnology
Elimination Tool:

A) dATP

B) Primers

C) DNA fragments

D) Ribonucleotides

65 / 67

Taq polymerase is extracted from a bacterium

Floral aquaticus

Taq aquaticus

Thermus floral

Thermus aquaticus

66 / 67

Liposomes have/are:

PMC 2021 Tested
Biology
Biotechnology
Elimination Tool:

A) Fluid in center

B) Lipid bilayer

C) All of these

D) Carrier for drugs

67 / 67

Bacterial cells take up recombinant plasmids when they are treated with:

UHS 2015
Biology
Biotechnology
Elimination Tool:

A) CaCl2

B) NaCl

C) KCl

D) NaOH

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Biology Biotechnology MCQs Chapter 9 Class 12

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